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February 05, 2012 06:55 AM
 
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CALCULATED CHANNELS

Sometimes measured detector response has to be preprocessed before it can be quantified. In such cases, creating artificial data channels using special data conversions from original data can prove a better approach.

Chrom&Spec supports several types of calculated channels that can be useful in a variety of circumstances

All examples refer to multi-channel chromatography, unless specially noted.

SUM OF SIGNAL-TO-NOISE RATIOS

Some peaks are clearly visible on one detector (WL) while other peaks are visible on another detector; the Sum of signal-to-noise ratios channel shows all of them. This channel can be very useful for peak detection (but unfortunately is not suitable for quantification).

AVERAGE CHANNEL

In the case of Diode Array detection (DAD) it’s helpful to use the average response for quantification purpose, e.g. in the range 240-260 nm. The main purpose of using the average channel is to increase s/n (signal-to-noise) ratio.

AVERAGE TRACE CHANNEL

To further improve s/n ratio and linear dynamic range, one can construct averaging regions with different averaging limits, appropriate to the set of substances being analyzed.

ANGLE

Shows angle derivative of the multi-channel chromatogram. The lower the angle, the higher the local purity of the peak point.

RESPONSE/TIME

 

This channel can be used for concentration-sensitive detectors and may improve area quantification results. It can compensate for analysis-to-analysis changes at flow and is especially useful in capillary electrophoresis. Response/Time can be used for single- or multi-channel detection.

FROM CALIBRATION POINT

This channel allows loading of calibration chromatogram channel to visually compare current and calibration chromatograms.
Other calculated channels are available

 

 

 

 

 

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